Journal: Journal of Traditional Chinese Medicine

Article Title: Tongqiao Yizhi granule (通窍益智颗粒) repress the nuclear factor kappa-b/nucleotide oligomerization domain-like receptors 3/caspase-1 pyroptosis pathway in the hippocampus to counter vascular dementia in rats

doi: 10.19852/j.cnki.jtcm.20240515.005

Figure Lengend Snippet: A: expression of caspase-1 protein in rat hippocampus (× 200); A1: Sham; A2: Model; A3: NMDP; A4: TQYZKL of 6.2 g·kg-1·d-1; A5: TQYZKL of 12.4 g·kg-1·d-1; A6: TQYZKL of 24.8 g·kg-1·d-1; B: expression of GSDMD protein in rat hippocampus (× 200), B1: Sham; B2: Model; B3: NMDP; B4: TQYZKL of 6.2 g·kg-1·d-1; B5: TQYZKL of 12.4 g·kg-1·d-1; B6: TQYZKL of 24.8 g·kg-1·d-1; Dyeing method of all pictures are the immunohistochemical method; C: expression of IL-18 and IL-1β in serum of rats. Sham and Model group: treated with physiological saline; NMDP group: treated with nimodipine (6.25 mg·kg-1·d-1); 6.2, 12.4, 24.8 group: treated with TQYZKL (6.2 g·kg-1·d-1), TQYZKL (12.4 g·kg-1·d-1), TQYZKL (24.8 g·kg-1·d-1) respectively. TQYZKL: Tongqiao Yizhi granule; NF-κB: nuclear factor kappa-B; NLRP3: nucleotide oligomerization domain-like receptors 3; IHC: immunohistochemistry; ELISA: enzyme-linked immunosorbent assay; IL-18: interleukin-18; IL-1β: interleukin-1β; NMDP: nimodipine. Data are presented as mean ± standard error of the mean (n = 4). Significant differences compared with Sham group were designated as aP < 0.05, with Model group as bP < 0.05 and with NMDP group with cP < 0.05.

Article Snippet: Anti-Golgi peripheral membrane protein p65 (P65) antibody (8242, CST, Boston, USA); anti-NLRP3 antibody (AF2155, Beyotime, Shanghai, China); anti-caspase-1 antibody (22915-1-AP, Proteintech, Chicago, IL, USA); Anti-Gasdermin D (GSDMD) antibody (AF4012, Affinity Biosciences, Cincinnati, OH, USA); anti-β-actin antibody (4967S, CST, Boston, MA, USA); horseradish peroxidase-conjugated anti-rabbit secondary antibody (7074S, CST, Boston, MA, USA); reverse transcription kit (R2028, UE, Beijing, China); real-time fluorescence quantitative polymerase chain reaction (PCR) kit (S2024, UE, Beijing, China); Tunel kit (C1090, Beyotime, Shanghai, China); Hematoxylin-eosin (HE) staining kit (C0105S, Beyotime, Shanghai, China); immunohistochemical staining kit (PV-9001, Zhongshan Golden Bridge, Beijing, China); enzyme-linked immunosorbent assay (ELISA) rat Interleukin-18 (IL-18) ELISA kit (ERC010.96, eBioscience, San Diego, CA, USA); ELISA rat Interleukin-1β (IL-1β) ELISA kit (ERC007.96, eBioscience, San Diego, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Saline, Immunohistochemistry, Enzyme-linked Immunosorbent Assay